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KMID : 0381920080380020125
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Korean Journal of Microscopy
2008 Volume.38 No. 2 p.125 ~ p.133
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The Formation and Change of Cytochrome-c-oxidase in the Mitochondria of the Bovine Cardiac Muscle
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Kim Soo-Jin
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Abstract
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Cytochrome-c-oxidase in mitochondria membrane is one of the most important factors for energy generation in the cell. As well as it is electron transfer enzyme, it is also heavily related to the apoptosis and other pathologic conditions.
Meanwhile, porin is a protein located in inner and outer membranes of mitochondria, which is assumed to be functionally
correlated with cytochrome-c-oxidase. It functions as forming electron transfer chain and conveying ATP. Therefore, using the immune-microscopy, It compared the distribution of cytochrome-c-oxidase and porin to figure out the formation and changes on cytochrome-c-oxidase in mitochondrial cristae. The sarcroplasm of cardic muscle tissue has many mitochondria. They are classified into two groups: the mitochondria with many cytochrome-c-oxidase and the itochondria with only porins. The mitochondria with porins had few cytochrome-c-oxidases in their membrane; in contrast, the other mitochondria with rich cytochrome-c-oxidase had few porins in their walls. In addition, according to the location of the tissue in bovine heart, distribution of those kind of mitochondria had been clearly separated. As a result, it could be assumed that immature mitochondria has many porins to transfer the protein materials from sarcroplasm through the porins, and they made cytochrome-c-oxidase until it is enough, and then they decreased the porin and maintained minimum number of the porin.
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KEYWORD
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Cytochrom-c-oxidase, Porin protein, Bovine cardiac muscle tissue Immunogold labeling, Immuno-florescence labeling
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